Figure 6.

Biophysical characterization of the full-length pre-miR-1229_SNP FL sequence. ¹H NMR spectra (A) and CD spectra (B) at various KCl concentrations in 10 mM cacodylic acid, pH 6.5 reveal an equilibrium between hairpin and GQ structures after annealing the RNA in 150 mM KCl. UV thermal denaturation hypochromic transition (C) at 10 µM RNA in 100 mM KCl was fit using Equation (1) (Materials and Methods), to determine a T_m of ~86 °C. Nondenaturing gel electrophoresis (D) visualized by UV shadow (left panel) and stained with the GQ-specific NMM dye (right panel), revealing the formation of multiple GQ structures as well as a hairpin structure.