Table 5.
Stability of SH-1242 and IS in stock solutions under typical storage conditions.
| Batch (n = 3) | Response (Peak Area) | |||||
|---|---|---|---|---|---|---|
| Initial (0 h) | Room Temp. (6 h) | Refrigerated (4 °C, 24 h) | Refrigerated (4 °C, 2 Weeks) | Refrigerated (−20 °C, 2 Weeks) | Refrigerated (−80 °C, 2 Weeks) | |
| SH-1242 a | ||||||
| Mean | 18,000 | 19,700 | 19,000 | 15,400 | 16,100 | 16,400 |
| CV(%) b | 3.93 | 13.5 | 11.1 | 9.14 | 5.44 | 3.01 |
| Relative conc. (%) c | 100 | 109 | 105 | 85.2 | 89.1 | 90.8 |
| IS | ||||||
| Mean | 19,100 | 19,200 | 20,000 | 17,600 | 17,400 | 16,400 |
| CV(%) | 4.27 | 6.20 | 0.762 | 4.65 | 8.36 | 1.53 |
| Relative conc. (%) | 100 | 101 | 105 | 92.3 | 91.4 | 86.2 |
a Stock solutions of SH-1242 were diluted to 50 ng/mL prior to analysis. b CV(%) = (standard deviation/mean) × 100. c Relative concentrations (%) were obtained by dividing measured values by initial values. IS: internal standard