Figure 5.

Effect of T and inhibitors treatment on cell cycle of myoblasts cultured in vitro. (A–B): Cell cycle of myoblasts treated with T and/or inhibitors (Bicalutamide, B; LY294002, LY; KP372-1, KP) was detected by flow cytometry. The percentage of S or G2/M stage cells was measured in different treatment compared with control (Con). A minimum of 15,000 myoblast cells were analysed for each sample. Values are means + SE of four independent experiments with triplicate determinations. Asterisk (*) represents statistically different (p < 0.05), ** indicates that the difference is extremely significant (p < 0.01).