Figure 5.

HMOX1 detection by quantitative real time polymerase chain reaction. Human bronchial epithelial 16HBE cells were maintained in control conditions (Ctlr) or treated with 16 or 32 g/cm of (A) titanium dioxide (TiO₂), (B) silica coated titanium dioxide (SiO₂TiO₂), (C) aluminum coated titanium dioxide (Al₂O₃TiO₂) or (D) double silica and aluminum coated titanium dioxide (AlSiTiO₂), and 4 Gy irradiated or not. ARN extractions were performed immediately (T0), 24 h, 48 h or 72 h after treatment and quantitative RT-PCR were realized to detect HMOX1 gene expression. Representative dot plots normalized by the value of the untreated non-irradiated control for each time point are shown (means ± S.D., n = 2). * p < 0.05, ** p < 0.01, *** p < 0.001 (Student’s t-test), as compared with the control group corresponding.