Figure 4.

(a) Schematic diagram of target search by p53 in the cell. p53 (pink spheres) may bypass other DNA-binding proteins (light blue squares) bound to DNA (black lines) using intersegmental transfer. (b) Single-molecule verification of intersegmental transfer of p53 between crisscrossing DNAs (left). The typical data of p53 demonstrating intersegmental transfer (right). (c) Schematic diagram of intersegmental transfer of p53. p53 binds to the first DNA mainly using the CT domain (Left), grabs the second DNA (Middle), and then releases the former while maintaining contact with the latter DNA (Right). The panels (a) and (c) are adapted from ref. [25] with some modifications, and the panel (b) is adapted from ref. [41] with some modifications.