Figure 4.

Tiam1 (T-lymphoma invasion and metastasis-1) expression of the different cell lines. (A) Representative images show Tiam1 (green) distribution. The non-transfected and scrambled cells exhibit an asymmetric, polarized Tiam1 arrangement, which is absent due to SDC4 silencing. Nuclei are blue (Hoechst 33258). Representative pseudocolor images (2D and 3D) depict Tiam1 signal intensity. The color was assigned to each pixel based on the pixel intensity value according to the calibration bar (shown on left-hand sides of the images). The mean intensity values in the individual cells are quantified in (B), and the average standard deviation values of the Tiam1 intensities are depicted in (C); n = 4–20 cells/cell line were analyzed; data are reported as mean+ SEM; **** p < 0.0001.