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. 2020 Jan 21;21(3):705. doi: 10.3390/ijms21030705

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© 2020 by the author.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Architecture of dual transcriptional-translational control systems. Blue and pink arrows indicate transcription and translation, respectively. (A) HYZEL. RF1, peptide release factor 1. (B) RiboTite(tT/tT). (C) cymO/R and theophylline riboswitch control. P21, synthetic P21 promoter. cmt, operator sequence of the cumate degradation operon. CymR, CymR regulator. TheoRS, theophylline riboswitch. (D) cymO/R and theophylline ribozyme control. TheoRZ, theophylline ribozyme. (E) Transcriptional terminator and occluded RBS control. P_repC, promoter regulating repC. TT + oRBS, transcriptional terminator and occluded RBS. repC-N, first 12 nucleotides of repC. (F) Transcriptional terminator and anti-toxin mRNA antisense RNA control. P_CON, a constitutive promoter. TT, transcriptional terminator. asTOX, antisense RNA against the RBS-start codon region of toxin gene mRNA.

Architecture of dual transcriptional-translational control systems. Blue and pink arrows indicate transcription and translation, respectively. (A) HYZEL. RF1, peptide release factor 1. (B) RiboTite(tT/tT). (C) cymO/R and theophylline riboswitch control. P21, synthetic P21 promoter. cmt, operator sequence of the cumate degradation operon. CymR, CymR regulator. TheoRS, theophylline riboswitch. (D) cymO/R and theophylline ribozyme control. TheoRZ, theophylline ribozyme. (E) Transcriptional terminator and occluded RBS control. P_repC, promoter regulating repC. TT + oRBS, transcriptional terminator and occluded RBS. repC-N, first 12 nucleotides of repC. (F) Transcriptional terminator and anti-toxin mRNA antisense RNA control. P_CON, a constitutive promoter. TT, transcriptional terminator. asTOX, antisense RNA against the RBS-start codon region of toxin gene mRNA.