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NE increases Nox2 and Nox4 mRNA expression. Rat lung microvascular endothelial cells (RLMEC) were stimulated with norepinephrine (NE, 5 ng/mL) or vehicle (V) for 4 to 24 hours (h). (a) NE does not alter mRNA expression of Nox1. (b) NE increases mRNA expression of Nox2 after 24 hours. (c) NE increased mRNA expression of Nox4 in a time-dependent manner. ∗p < 0.05 vs. vehicle (V). N = 5/group.
