Table 2.
Results of the experiment performed to determine the LOQ of the GC-MS method for acetazolamide (AZM) in human urine.
| Added AZM (μM) | PAR 581/584 | Measured AZM (μM) | Precision (RSD, %) | Recovery (%) | PAR 83/86 | Measured AZM (μM) | Precision (RSD, %) | Recovery (%) |
|---|---|---|---|---|---|---|---|---|
| 0 | 0.006 ± 0.004 | 1.57 ± 1.05 | 66.9 | not applicable | 0.008 ± 0.002 | 2.26 ± 0.57 | 25.2 | not applicable |
| 25 | 0.121 ± 0.006 | 31.7 ± 1.57 | 5.0 | 120.5 | 0.114 ± 0.008 | 32.3 ± 2.26 | 7.0 | 120 |
| 50 | 0.217 ± 0.007 | 56.9 ± 1.83 | 3.2 | 110.7 | 0.200 ± 0.004 | 56.6 ± 1.13 | 2.0 | 108.7 |
| 100 | 0.396 ± 0.010 | 103.8 ± 2.62 | 2.5 | 102.2 | 0.367 ± 0.019 | 103.9 ± 5.4 | 5.2 | 101.6 |
| 150 | 0.593 ± 0.011 | 155.4 ± 2.88 | 1.9 | 102.6 | 0.550 ± 0.015 | 155.7 ± 4.2 | 2.7 | 102.3 |
| 200 | 0.777 ± 0.019 | 203.6 ± 5.0 | 2.5 | 101.0 | 0.723 ± 0.047 | 204.6 ± 13.3 | 6.5 | 101.2 |