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. 2020 Jan 31;21(3):959. doi: 10.3390/ijms21030959

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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AMPK/mTORC1 signaling pathway was activated and act as a checkpoint during ColXV activating adipocytes apoptosis. (A–B) Relative protein phosphorylation levels of AMPKAα1^Thr183, AMPKAα2^Thr172 and mTORC1^Ser2448 in different groups with or without Compound C (C.C) treatment (n = 3). (C–D) Relative protein levels of Caspase-3/Cleaved Caspase-3 and Bcl-2 in different groups with or without Compound C (C.C) treatment (n = 3). (E–F) Relative protein phosphorylation levels of mTORC1^Ser2448 and S6K1^Thr389 in different groups with or without rapamycin (Rapa) treatment (n = 3). (G–H) Relative protein levels of Caspase-3/Cleaved Caspase-3 and Bcl-2 in different groups with or without rapamycin (Rapa) treatment (n = 3). (I–J) Tunnel staining of DNA fragments in adipocytes with or without Compound C (C.C) treatment. Scale bar, 100 μm (n = 4). Values are presented as mean ± SEM. *P < 0.05, ** P < 0.01.