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. 2020 Jan 25;21(3):785. doi: 10.3390/ijms21030785

Figure 7.

Figure 7

Multiple disease phenotype mediated by osa-miR159b, osa-miR164a and osa-miR167d-5p in overexpressing transgenic plants. (A–C) The excessive accumulation of osa-miR159b, osa-miR164a and osa-miR167d-5p in overexpressing transgenic lines. The wild-type (WT) Taipei 309 was used as control. (D and E) Diverse disease phenotypes caused by the Xoo strain PXO86 in different overexpressing transgenic lines. Lesion lengths for 45 diseased leaves in 15 plants were statistically analyzed at 14 dpi. (F) The histochemical detection of H2O2 using 3,3′-diaminobenzidine (DAB) staining in WT and overexpressing transgenic lines after Xoo infection. Scale bar, 1 cm. (G–I) The relative expression levels of targets in overexpressing transgenic lines. Bars represent mean ± standard deviation (n = 3 independent biological replicates). Asterisks indicate a statistically significant difference (student t-test, ** p < 0.01, * p < 0.05).