Figure 5.

Effects of sodium selenite on estradiol levels in heat stress-treated granulosa cells and serum in mouse. Cells were treated with or without sodium selenite at 39 °C for 24 h and then the concentration of estradiol in the culture supernatants was detected by an ELISA kit (A). Western blotting was used to analyze the protein expression of CYP19A1 (B and C). β-actin was used to normalize the protein expression levels. The mouse was orally administered sodium selenite for 4 weeks, and the estradiol level in the serum was detected by an ELISA kit (D). The statistical analysis results are shown as bar graphs. The data are represented as the mean ± SEM of three independent experiments, and each independent experiment includes three technical replicates. Bars with different lowercase letters are significantly different (p < 0.05).