Table 4.
scFc analysis via RP-HRMS. Average experimental masses were determined based on triplicate analysis, as well as mass accuracies and relative abundancies. C-term lysine clipping was considered in the theoretical mass calculation unless indicated otherwise.
| mAbs | Modifications/Glycoforms | Experimental mass (Da) | Theoretical monoisotopic mass (Da) | Mass difference (ppm) | Relative abundance (%) |
|---|---|---|---|---|---|
| BEV | Unglycosylated | 23775.974 | 23775.930 | 1.9 | 1.4 |
| A2G0F | 25220.485 | 25220.463 | 0.8 | 84.9 | |
| A2G1F | 25382.504 | 25382.516 | 0.5 | 10.3 | |
| A1G0F | 25018.436 | 25017.380 | 2.3 | 1.9 | |
| A2G0 | 25074.482 | 25074.410 | 2.9 | 1.5 | |
| TRA | A2G0 | 25074.431 | 25074.405 | 1.0 | 4.3 |
| A2G0F | 25220.513 | 25220.463 | 2.0 | 52.0 | |
| A2G1F | 25382.581 | 25382.516 | 2.6 | 36.2 | |
| A1G0F | 25017.445 | 25017.380 | 2.6 | 1.8 | |
| M5 | 24992.715 | 24992.350 | 14.6 | 1.6 | |
| A2G2F | 25544.569 | 25544.569 | 0.0 | 4.1 | |
| INF | M5 | 24960.391 | 24960.380 | 0.4 | 1.6 |
| A1G0F | 24985.423 | 24985.412 | 0.4 | 2.4 | |
| 1x C-term lysine, M5 | 25088.477 | 25088.475 | 0.1 | 1.8 | |
| 1x C-term lysine, A2G0F | 25316.594 | 25316.586 | 0.3 | 38.6 | |
| 1x C-term lysine, A2G1 | 25332.552 | 25332.581 | 1.1 | 1.4 | |
| 1x C-term lysine, A2G0 | 25170.536 | 25170.528 | 0.3 | 3.0 | |
| A2G0F | 25188.488 | 25188.487 | 0.0 | 30.2 | |
| A2G1F | 25350.558 | 25350.544 | 0.6 | 10.9 | |
| 1x C-term lysine, A2G1F | 25478.629 | 25478.639 | 0.4 | 10.1 | |
| RIT | A2G0F | 25188.541 | 25188.491 | 2.0 | 44.2 |
| A2G1F | 25350.583 | 25350.544 | 1.5 | 48.3 | |
| A2G2F | 25512.604 | 25512.597 | 0.3 | 7.5 |