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. 2020 Feb 7;25(3):728. doi: 10.3390/molecules25030728

Figure 4.

Figure 4

ORN-1 released from RDgel prepared with different metal ions. RDgel prepared with 30, 150, or 750 mM sodium ions, or with 2, 10, 50 mM magnesium ions was used. (a) Time course of the amount of RNA/DNA in RDgel prepared with sodium ions remaining on the Transwell chamber. The amount of RNA/DNA at 1, 3, 6, 12, and 24 h after RNA/DNA addition was calculated by subtracting the released amount from the total amount. (b) Time course of FAM-ORN-1 released from RDgel prepared with sodium ions. The fluorescence intensity of PBS in the lower chamber was measured at 1, 3, 6, 12, and 24 h after starting the experiment. (c) Time course of the amount of RNA/DNA in RDgel prepared with magnesium ions remaining on the Transwell chamber. The amount of RNA/DNA at 1, 3, 6, 12, and 24 h after RNA/DNA addition was calculated by subtracting the released amount from the total amount. (d) Time course of FAM-ORN-1 released from the RDgel prepared with magnesium ions. The fluorescence intensity of PBS in the lower chamber was measured at 1, 3, 6, 12, and 24 h after starting the experiment. The results are expressed as mean ± SD of three independent experiments, * p < 0.05 compared with 30 mM sodium ions or 2 mM magnesium ions.