Figure 7.

Schematic representation of carbon-nitrogen metabolism changes in non-grafted cucumber (A) and rootstock grafted cucumber (B) exposed to CA stress. Photosynthesis, Calvin cycle, glycolysis, TCA cycle, glyoxylate cycle and nitrogen metabolism were regulated by grafting. Different color indicates level of gene expression with red representing up-regulation while green as down-regulation. The level of regulation was determined based on log₂ fold change. The blue arrow represents the direction of substance metabolism in cyclic metabolic pathway. [Abbreviations for different subunits: PSII reaction centre core protein (PsbY, PsbW, Psb27, Psb28); subunit of oxygen evolving complex protein (PsbP); oxygen-evolving enhancer protein (PsbQ); PSI reaction center subunit N (PsaN); PSI reaction center subunit III (PsaF); sex-linked protein 9 (PsaO); Ferredoxin (Fd); Plastocyanin (PC); Abbreviations for enzymes: ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisco); glyceraldehyde 3-phosphate dehydrogenase (GAPDH); transketolase (TKL); ribose-5-phosphate isomerase (RPI); phosphoribulokinase (PRK); hexokinase (HK); phosphoglucose isomerase (PGI); phosphofructokinase (PFK); aldolase (ALL); phosphoglycerate kinase (PGK); phosphoglycerate mutase (PGM); enolase (ENL); pyruvate kinase (PK); pyruvate dehydrogenase (PDH); phosphoenolpyruvate carboxylase (PEPC); citrate synthase (CS); isocitrate lyase (ICL); malate synthase (MAS); malate dehydrogenase (MADH); glutamate dehydrogenase (GDH); glutamate synthase (GOGAT); glutamine synthetase (GS); nitrite reductase (NiR), nitrate reductase (NR); Abbreviations for metabolites: ribulose biphosphate (RuBP), 3-phosphoglycerate (3-PG), 1,3-bisphosphoglycerate (1,3-BPG); G-3-P, ribose 5-phosphate (R-5-P); ribulose5-phosphate (Ru-5-P); glucose 6-phosphate (G-6-P); fructose 6-phosphate (F-6-P); fructose 1,6-bisphosphate (F-1,6-BP); 2-phosphoglycerate (2-PG); phosphoenolpyruvate (PEP); oxaloacetate (OAA); 2-oxoglutarate (2-OG); glutamate (Glu); glutamine (Gln)].