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. 2020 Feb 10;25(3):757. doi: 10.3390/molecules25030757

Figure 4.

Figure 4

EMT-activating transcription factors were detected using Western blot and immunofluorescence. (A) The Western blot showed that quercetin downregulated EMT transcription factors at the protein level. (B) Quantitation of A. The band intensities of each target protein were measured using an image analyzer and presented as relative ratio. (C) Representative fluorescence microscopy images of Twist in OSCC cell lines. (D) Representative fluorescence microscopy images of Slug in OSCC cell lines. Scale bars: 20 μM. Data are the means ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. the corresponding control (quercetin 0 μM).