Figure 5.

Quercetin was not toxic in normal keratocyte and transforming growth factor β1 (TGF-β1) stimulated EMT in HaCaT cells. (A) To examine the effect of quercetin on cell viability, an MTT assay was conducted on HaCaT and nHOK cells. Data are means ± SEM. * p < 0.05 vs. the corresponding control (quercetin 0 μM). (B) Expression of EMT-related markers such as in TGF-β1-treated HaCaT cells were analyzed by Western blotting. (C–H) Quantitation of B. The band intensities of each target protein were measured using an image analyzer and presented as relative ratio. Data are means ± SEM. * p < 0.05 and *** p < 0.005 vs. corresponding control (TGF-β1 0 ng/mL).