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. 2020 Jan 26;21(3):805. doi: 10.3390/ijms21030805

Figure 1.

Figure 1

DPP-4 suppression enhanced the expression of ATP-binding cassette (ABC) transporters in breast cancer cells treated with chemotherapy drugs. (A) Western blot analysis of P-gp and ABCG2 in 4T1 cells pretreated with KR62436 (50 μmol/L) for 48 h and then treated with or without doxorubicin (DOX;0.425 μmol/L) for another 48 h. (B) Western blot analysis of P-gp and ABCG2 in DPP-4 shRNA knockdown (DPP-4-kd) and shRNA control (control) 4T1 cells treated with or without DOX (0.425 μmol/L) for 48 h. (C,D) Western blot analysis of MRP1 and ABCG2 in MCF-7 cells (C) and MDA-MB-231 cells (D) pretreated with KR62436 (50 μmol/L) for 48 h and subsequently treated with or without DOX (0.425 μmol/L) for another 48 h. All representative blots from three independent experiments were shown, and densitometric analysis of protein expression relative to β-actin levels was performed by using ImageJ.