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. 2020 Jan 26;21(3):805. doi: 10.3390/ijms21030805

Figure 2.

Figure 2

DPP-4 inhibition increased ABC transporters in combination with chemotherapeutic insult in a CXCL12/CXCR4/mTOR/TGF-β-dependent manner in breast cancer cells. (A,B) Western blot analysis of P-gp and ABCG2 in 4T1 cells pretreated with KR62436 (50 μmol/L) for 48 h and then treated with DOX (0.425 μmol/L) in the presence or absence of AMD3100 (30 μmol/L) (A) and rapamycin (1 μmol/L; B) for another 48 h. (C,D) Western blot analysis of mTOR phosphorylation (P-mTOR), P-gp, ABCG2 and snail in 4T1 cells (C) and MDA- MB-231 cells (D) pretreated with KR62436 (50 μmol/L) for 48 h and then treated with DOX (0.425 μmol/L) in the presence or absence of a neutralizing TGFβ (1, 2 and 3) antibody (N-TGFβ, 1.0 μg/mL) for another 48 h. All representative blots from three independent experiments were shown, and densitometric analysis of protein expression relative to β-actin levels was performed using ImageJ.