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. 2020 Jan 26;21(3):805. doi: 10.3390/ijms21030805

Figure 4.

Figure 4

DPP-4 inhibition protects breast cancer cells from apoptosis. (A–C) Detection of early apoptosis utilizing flow cytometry (annexin V-FITC apoptosis staining) in 4T1 cells pretreated with KR62436 (50 μmol/L) for 48 h and then treated with or without doxorubicin (0.425 μmol/L; A) or docetaxel (DOC; 0.9 μmol/L; B) for another 24 h in the presence or absence of the neutralizing TGF-β (1, 2 and 3) antibody (N-TGFβ, 1.0 μg/mL; C) for another 24 h. Densitometric analysis of early apoptotic cells (%) in each group (n = 6 per group). (D) Western blot analysis of cleaved caspase-3 in 4T1 cells pretreated with KR62436 (50 μmol/L) for 48 h and then treated with or without DOX (0.425 μmol/L) for another 48 h. (E–G) Western blot analysis of 4T1 cells pretreated with KR62436 (50 μmol/L) for 48 h and subsequently treated with or without DOX (0.425 μmol/L) in the presence or absence of the neutralizing TGFβ (1, 2 and 3) antibody (N-TGFβ, 1.0 μg/mL; E), AMD3100 (30 μmol/L; F), or rapamycin (1 μmol/L; G) for another 48 h. All densitometric analyses of protein expression relative to the caspase3 levels (n = 3 per group) were performed by using ImageJ.