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. 2020 Feb 3;21(3):999. doi: 10.3390/ijms21030999

Figure 3.

Figure 3

miR-21-5p mimic transfection suppressed IHR-induced cytotoxicity/apoptosis, and inhibit IHR-induced upregulations of several predicted target genes, including TNF-α, HIF-2α, NFAT5, ELF2, IL6, IL6R, EDNRB, and TLR4. miR-21-5p transfection (a) enhanced miR-21-5p expression and suppressed (b) TNF-α, (c) TLR4, and (d) IL6R gene expressions under normoxic (NOX) condition in a dose-dependent manner. Intermittent hypoxia with re-oxygenation (IHR) augment (e) cytotoxicity, (f) early apoptosis, and increased gene expressions of the (g) TNF-α, (h) HIF2-α, (i) NFAT5, (j) ELF2, (k) IL6, (l) IL6R, (m) EDNRB, and (n) TLR4 genes, while all of these changes were reversed with miR-21-5p transfection under IHR condition. * p < 0.05, compared with 0 nM miR-21-5p mimic or NOX condition. ** p < 0.01, compared between IHR and NOX condition by Mann-Whitney test. # p < 0.05, compared with IHR alone condition.