Figure 4.

Ang-(1-7) reduced the reactive oxygen species (ROS) production induced by myostatin in the myotubes. (A) C₂C₁₂ myotubes were incubated with myostatin (Mstn, 1 µg/mL) for 24 h in the presence or absence of Ang-(1-7) (10 nM; pre-incubated for 1 h). The ROS levels were then determined by the detection of fluorescence using a CM-DCF-DA probe. The nuclei were labelled with Hoechst. The scale bar represents 100 µm. (B) Quantification of the DCF-positive myotubes. The values are expressed as a fold of induction relative to the myotubes without treatment, and correspond to the mean ± SD from three independent experiments (n = 3; * p < 0.05 vs. control without treatment).