Figure 5.

Ang-(1-7) reduced the myostatin-induced NF-κB signaling in myotubes. (A) C₂C₁₂ myotubes were pre-incubated with or without Ang-(1-7) (10 nM) and after 1 h with myostatin (Mstn, 1 µg/mL) for 24 h. Protein levels of IκB and β-actin were detected by Western blot analysis. Molecular weights are shown in kDa. (B) Quantitative analysis for IκB protein levels with values normalized to β-actin. (C) Luciferase activity of pNF-κB-luc. (D) The mRNA levels of TNF-α were determined by RT-qPCR. The values are expressed as a fold of induction relative to the control (myotubes without treatment), and correspond to the mean ± SD from three independent experiments (n = 3; * p < 0.05 vs. control without treatment).