Figure 6.

Reduction of myostatin dependent on muscle atrophy and signaling by Ang-(1-7) is mediated through Mas receptor in myotubes. C₂C₁₂ myotubes, pretreated with or without Ang-(1-7) (10 nM), A779 (10 µM) (pre-incubated for 30 min), or both, were incubated with myostatin (Mstn, 1 µg/mL) for 12 or 24 h. The parameters measured were: (A) distribution of the myotube diameters; mRNA levels of (B) atrogin-1, (C) MuRF-1, and (E) TNF-α; (D) Luciferase activity of pNF-κB-luc; and (F) ROS levels. The values are expressed as a fold of induction relative to the myotubes without treatment and correspond to the mean ± SD from three independent experiments (n = 3; * p < 0.05 vs. control without treatment).