Figure 2.

Apoptosis in Jurkat and Jurkat/A4 cells under simulated microgravity (96 h). Cells were stained with annexin V, conjugated, and evaluated for apoptosis as described in the Materials and Methods section. (a,c) Flow cytometric analysis of cells to assess apoptosis using annexin V labelling. Results are shown as percentages of viable cells (annexin V−/propidium-iodide (PI)−), early apoptotic cells (annexin V+/PI−), late apoptotic cells (annexin V+/PI+), and dead cells (annexin V−/PI+). The apoptosis rates were statistically evaluated. (b,d) Quantitative comparison of apoptosis between the static control and RPM groups. The results are expressed as means ± standard deviations. *p < 0.05, as compared with the static controls (n = 3).