Figure 1.

ZIP4 Zn²⁺ transport assay. (A) Immunoblot analysis (Lower panel) and normalized surface expression by densitometry (upper panel) of HEK293-T cells transfected with hemagglutinin (HA)-tagged mZIP4 and exposed to 20 µM Zn²⁺ for the indicated times N ≥ 5. (B) Experimental assay used to monitor Zn²⁺ uptake in HEK293-T cells transfected with an empty control vector (black) or mZIP4 (blue) N ≥ 5. Cells were loaded with 1 µM Fluozin-3AM and subjected to 50 µM Zn²⁺ and 20 µM TPEN, as indicated.