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. 2020 Jan 22;21(3):734. doi: 10.3390/ijms21030734

Figure 5.

Figure 5

Genetic variations in ZIP4 affect catalytic and non-catalytic domains. (A) Immunoblot analysis of Acrodermatitis enteropathica (AE)-associated ZIP4 constructs, as indicated, from HEK293-T cell lysates. (B) Membrane orientation of ZIP4 illustrates the position of AE-associated variants P200L and G539R. (C) Normalized rates of Zn2+ uptake at pH7.4 (bottom panel—striped) and pH5 (top panel—filled), mediated by AE-associated ZIP4 mutants, in HEK293-T cells loaded with Fluozin-3AM N ≥ 5. (D) Normalized rates of H+ uptake at pH 7.4 (bottom panel—striped) and pH5 (top panel—filled), mediated by AE-associated ZIP4 mutants, in HEK293-T cells loaded with BCECF-AM. (E–F) Representative traces of Zn2+ (red) and H+ uptake (blue) recorded in HEK293-T cells transfected with ZIP4P200L (E) and ZIP4G539R (F) n ≥ 3. In (C) and (D), # is p ≤ 0.05 between cells expressing ZIP4 and G539R mutant at pH5.