Table 1.

Site directed mutagenesis reaction components.

Component	Amount
dsDNA template (100 ng)	0.333 µL
Primer F (0.3 µM)	0.75 µL
Primer R (0.3 µM)	0.75 µL
dNTPs	1.5 µL
Enzyme	0.5 µL
ddH20 (a total of 25 µL)	21.167 µL
Total volume	25 µL