Table 2.

Site directed mutagenesis PCR.

Cycles	Step	Temperature	Time (min:s)
1	Initial denaturation	95 °C	5:00
20	Denaturation	98 °C	0:20
	Annealing	78 °C	0:20
	Elongation	72 °C	0:30/1 kb template
1	Final elongation	78 °C	5:00