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. 2020 Jan 22;21(3):734. doi: 10.3390/ijms21030734

Table 2.

Site directed mutagenesis PCR.

Cycles Step Temperature Time (min:s)
1 Initial
denaturation
95 °C 5:00
20 Denaturation 98 °C 0:20
Annealing 78 °C 0:20
Elongation 72 °C 0:30/1 kb
template
1 Final
elongation
78 °C 5:00