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. 2020 Jan 28;21(3):834. doi: 10.3390/ijms21030834

Figure 2.

Figure 2

MME mRNA and protein in fpEC after normal and overweight pregnancy. MME mRNA (A), cellular protein (B), and released MME (C) was reduced in primary fpEC exposed to maternal overweight (t-test). When correlated to maternal pre-pregnancy BMI (Pearson correlation), this association was not significant for fpEC MME mRNA (D), but it was significant for fpEC protein production (E) and release (F). MME mRNA was normalized to the mean of the housekeeping genes HPRT1 and ribosomal protein L30 (RPL30), respectively. A representative immunoblot for MME, β-actin, and the Ponceau S staining of the corresponding membrane are shown on top of the protein data in (B). C: controls; OW: overweight; a.u.: arbitrary units. MME mRNA: n(c) = 19; n(ow) = 15; cellular MME: n(c) = 19; n(ow) = 11; MME release: n(c) = 10; n(ow) = 7. The bars in (A–C) represent the mean ± SEM.