Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Feb 3;21(3):1002. doi: 10.3390/ijms21031002

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Involvement of cholesterol biosynthesis pathway in BIX-induced cell death. (A–C) Expression of SREBF2 (A), HMGCR (B), and HMGCS1 (C) was analyzed in BIX- and 25-hydroxycholesterol (25-HC)-treated H1299 cells. (D) MTT assay was conducted in lysates of H1299 with BIX and 25-HC treatment, based on 0 μM of BIX and 25-HC as 100%. (E,F) Expression of the autophagy-related genes ATG5 (E) and MAP1LC3B (F) was analyzed in BIX- and 25-HC-treated H1299 cells. Data were normalized by the value of 0 μM BIX in each 25-HC treatment. (G) LC3B protein levels were analyzed by Western blotting after BIX and 25-HC treatment for 48 h in H1299 cells. α-Tubulin levels are shown as loading control. Relative intensities of LC3B-II bands against a-tublin were quantified and presented in the right panel. * p < 0.05 versus 0 μM BIX treatment. # p < 0.05 versus 2.5 μM 25-HC treatment.