Fragments from different regions of 5S rRNA accumulated in piwi mutants are different in size: Hybridization of one blot with each of three probes detecting the 5′-terminus, middle and 3′-terminus of the 120 n. 5S rRNA was carried out by sequential reprobing and the results are presented in three different signal intensities increasing from left to right, separated by thick black lines. In the beginning of the molecule prominent fragments of ~70 and 80 n., as well as weak fragments of ~19, 21, 24 n., are observed, marked by squared white arrows. In the middle of the molecule major ~70 and 80 n. fragments are marked. In the end of the molecule prominent ~27, 28, and 29 n. fragments are marked. The wt genotype did not contain a mutation in piwi (nos-GAL4 driver line).