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. 2020 Feb 7;21(3):1119. doi: 10.3390/ijms21031119

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© 2020 by the author.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Mutations in other primary piRNA processing components, but not ping-pong components of the piRNA pathway, cause a significant accumulation of rRNAs fragments: (A) Effects of mutations in zuc and armi of primary piRNA processing. The fragments significantly accumulate in the transheterozygous zuc^HM27/Df(2L)Prl ovaries (exemplified by 5.8S rRNA on the left) and armi^1/72.1 ovaries (exemplified by 5.8S rRNA in the middle and 18S rRNA on the right). Fragments are designated by white arrows, mature rRNAs by black arrows; (B) Effects of mutations in aub and spn-E of ping-pong amplification. The fragments do not significantly accumulate in transheterozygous spn-E^616/3987 ovaries (exemplified by 5S rRNA on the left and in the middle and 18S rRNA on the right) and aub^QC42/HN2 ovaries (exemplified by 5S rRNA in the middle). Equal amounts of RNA were loaded on lanes of controls and mutants to be compared in each blot.

Mutations in other primary piRNA processing components, but not ping-pong components of the piRNA pathway, cause a significant accumulation of rRNAs fragments: (A) Effects of mutations in zuc and armi of primary piRNA processing. The fragments significantly accumulate in the transheterozygous zuc^HM27/Df(2L)Prl ovaries (exemplified by 5.8S rRNA on the left) and armi^1/72.1 ovaries (exemplified by 5.8S rRNA in the middle and 18S rRNA on the right). Fragments are designated by white arrows, mature rRNAs by black arrows; (B) Effects of mutations in aub and spn-E of ping-pong amplification. The fragments do not significantly accumulate in transheterozygous spn-E^616/3987 ovaries (exemplified by 5S rRNA on the left and in the middle and 18S rRNA on the right) and aub^QC42/HN2 ovaries (exemplified by 5S rRNA in the middle). Equal amounts of RNA were loaded on lanes of controls and mutants to be compared in each blot.