Figure 3.

Effect of β-caryophyllene on apoptosis of AGS cell infected with H. pylori. AGS cells were infected with H. pylori (200 MOI) and treated with indicated dose of β-caryophyllene (250 and 500 μg/mL) for 24 h. After incubation, the cells were stained with annexin V-FITC and PI and subjected to flow cytometry. β-caryophyllene alleviated apoptosis of AGS cells induced by H. pylori infection. (A) Stained cells were analyzed and illustrated on the quadrant by CellQuestPro software. (B) The percentage of cells in apoptosis was analyzed and illustrated as a graph. Results from triplicate experiments were analyzed by Student’s t-test (*** p < 0.001).