Table 1.
Percentage of HBC and THP-1 macrophages positive for indicated polarization markers upon M1 and M2 stimulation in comparison to untreated cells. For each cell type, five individual experiments were conducted. In the case of HBCs the five individual experiments equal five different cell isolations, i.e., donors. 2-way ANOVA with Dunnett’s post-hoc test was used to test statistical significance.
| HBC% pos. Population | THP-1% pos. Population | |||||||
|---|---|---|---|---|---|---|---|---|
| Untreated | LPS + INF-γ | IL-4 + IL-13 | p-Value | Untreated | LPS + INF-γ | IL-4 + IL-13 | p-Value | |
| CD163 | 82.9 ± 13.7 | 67.7 ± 31.5 | 79.0 ± 11.5 | 70.3 ± 24.7 | 91.5 ± 4.9 | 78.0 ± 18.4 | ||
| CD206 | 76.7 ± 18.4 | 27.6 ± 6.6** | 81.0 ± 4.7 | ** 0.01 | 2.7 ± 1.5 | 6.2 ± 0.7* | 2.7 ± 1.7 | *0.02 |
| DC-SIGN | 77.7 ± 11.4 | 26.9 ± 16.4* | 93.1 ± 2.7 | * 0.03 | 41.4 ± 10.5 | 34.7 ± 15.6 | 57.0 ± 32.2* | *0.01 |
| CD80 | 40.4 ± 20.4 | 32.8 ± 14.0 | 42.0 ± 20.2 | 4.9 ± 3.5 | 46.9 ± 18.5* | 3.2 ± 1.9 | *0.03 | |
| CD86 | 67.6 ± 18.1 | 50.1 ± 14.0 | 72.8 ± 11.3 | 24.8 ± 8.1 | 61.2 ± 19.7* | 23.1 ± 9.8 | *0.02 | |
| TLR1 | 31.4 ± 24.4 | 26.6 ± 11.3 | 34.7 ± 19.3 | 14.1 ± 5.7 | 16.1 ± 5.3 | 7.7 ± 3.6* | *0.04 | |
| TLR4 | 96.0 ± 3.6 | 84.6 ± 13.1 | 91.7 ± 4.3 | 74.3 ± 14.1 | 93.2 ± 12.0 | 66.3 ± 16.5 | ||
| HLA-DR | 74.8 ± 16.1 | 62.8 ± 12.8 | 77.5 ± 10.4 | 64.9 ± 13.7 | 95.8 ± 7.2** | 43.8 ± 8.9* | **0.005; *0.025 | |
| VEGF | 94.4 ± 6.5 | 88.8 ± 7.9 | 92.1 ± 7.8 | 96.8 ± 1.8 | 99.3 ± 0.5 | 96.8 ± 2.5 | ||