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. 2020 Feb 7;21(3):1112. doi: 10.3390/ijms21031112

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Development and application of gene-tagged molecular marker. (A) derived Cleaved Amplified Polymorphic Sequence (dCAPS) marker development for PpTST1. The G/T single nucleotide polymorphism (SNP) is highlighted in bold. The underlined character indicates the mismatched base for introducing the dCAPS marker. (B) genotyping of peach cultivars with the dCAPS marker. PCR products of each cultivar was digested with PstI and then separated on agarose gel. 1, PANDANA; 2, Diwang 1; 3, Gubatiantao; 4, Hongburuan; 5, 97-1-1; 6, Jinxiayoupantao; 7, Frederica; 8, Hanyin 22; 9, GHETA; 10, Asama Hakuto; 11, Lianhuang; 12, Zheng 97-4-51; 13, 4-3-3; 14, 99-13-9; 15, Xiahui 6; 16, Zhongyoupantao 2; 17, Meiguowanyou; and 18, Ruiguang 19. M, DNA ladder.