Figure 4.

Determination of adhesion molecule affected by dapagliflozin HCT116 cells were incubated with either vehicle (DMSO) or 0.5 mM dapagliflozin for 35 min and added to tissue culture dishes coated with bovine serum albumen (BSA), collagens I and IV, fibronectin, vitronectin, or laminin. After washing, the number of cells remaining and attached to the substratum was determined as described in the Experimental procedures section (n = 3, DMSO-treated cells vs. cells treated with 0.5 mM dapagliflozin * p < 0.0001, ** p < 0.001). The open column represents cells treated with 0.5 mM dapagliflozin and the closed column represents DMSO-treated cells. The experiments were conducted independently in triplicate.