Table 3.
Biochemical parameters in serum (and liver only for TBARS activity) for adult wild-type, Mpst (1st–3rd)-KO, Cth-KO, and Mpst (1st)/Cth-DKO mice.
| Mpst | Mpst (1st)/Cth | |||||
|---|---|---|---|---|---|---|
| Mice | WT (a) | 1st KO (b) | 2nd KO | 3rd KO | Cth-KO (c) | -DKO (d) |
| Serum | (8) | (7) | (7) | (7) | (5) | (10) |
| Albumin (g/dL) | 2.10 ± 0.12 | 2.21 ± 0.18 d | 2.14 ± 0.10 | 2.19 ± 0.20 | 2.00 ± 0.19 | 1.97 ± 0.16 b |
| ALT (IU/L) | 17.5 ± 10.8 | 12.1 ± 2.2 d | 16.3 ± 5.0 | 16.0 ± 5.3 | 22.0 ± 8.1 | 29.2 ± 12.6 b |
| AST (IU/L) | 45.3 ± 9.7 | 39.6 ± 12.3 d | 42.6 ± 16.6 | 42.1 ± 14.0 | 51.0 ± 20.4 | 75.2 ± 40.4 b |
| BUN (mg/dL) | 25.2 ± 3.3 | 25.4 ± 3.4 | 25.8 ± 1.9 | 26.8 ± 5.3 | 26.5 ± 2.5 | 26.5 ± 3.1 |
| CPK (IU/L) | 394 ± 110 | 306 ± 96 d | 448 ± 222 | 271 ± 62 | 351 ± 84 | 470 ± 154 b |
| CRE (mg/dL) | < 0.2 | < 0.2 | < 0.2 | < 0.2 | < 0.2 | < 0.2 |
| LDH (IU/L) | 173 ± 48 d | 157 ± 16 d | 282 ± 93 | 184 ± 28 | 225 ± 54 | 308 ± 80 ab |
| T-bilirubin (mg/dL) | 0.29 ± 0.11 | 0.30 ± 0.12 | 0.37 ± 0.11 | 0.27 ± 0.08 | 0.36 ± 0.11 | 0.35 ± 0.07 |
| T-protein (g/dL) | 4.59 ± 0.22 d | 4.71 ± 0.44 d | 4.39 ± 0.23 | 4.63 ± 0.49 | 4.26 ± 0.27 | 4.13 ± 0.23 ab |
| UA (mg/dL) | 2.81 ± 0.79 cd | 1.97 ± 1.12 | 1.74 ± 0.60 | 2.17 ± 0.79 | 1.60 ± 0.39 a | 1.63 ± 0.40 a |
| (6) | (5) | (6) | (8) | |||
| TBARS (nmol/mL) | 1.68 ± 0.22 | 1.68 ± 0.23 | N.T. | N.T. | 1.87 ± 0.41 | 1.50 ± 0.17 |
| Liver | ||||||
| TBARS | (6) | (5) | (6) | (8) | ||
| (nmol/µg protein) | 522 ± 29 d | 546 ± 77 | N.T. | N.T. | 659 ± 78 | 702 ± 169 a |
Means ± SD are presented (n: sample numbers). N.T., not tested. All parameters were similar in Mpst-KO mice lines; therefore, statistical analyses were done between WT, Mpst (1st)-KO, Cth-KO, and Mpst (1st)/Cth-DKO mice by one-way ANOVA with Tukey’s multiple comparison test. Significant differences (p < 0.05) versus WT (a), Mpst (1st)-KO (b), Cth-KO (c), and Mpst (1st)/Cth-DKO mice (d).