Figure 9.

Aβ40 and Aβ42 ELISA. We measured Aβ40 and Aβ42 levels in cell media after treatment with CuONP at 100 μM and L-BSO at 1 μM, and they were also measured when cells were pretreated with NAC for 1 h, followed by treatment of CuONP and L-BSO, respectively. Measurements were taken of SY5Y(A) levels for Aβ40 (A.1) and Aβ42 (A.2) and of H4 levels for Aβ40 (B.1) and Aβ42 (B.2). Aβ40 and Aβ42 levels were increased by treatment with CuONP, and only Aβ42 was increased by treatment with L-BSO on SH-SY5Y cells; Aβ40 level was increased by treatment with CuONP and L-BSO, but not Aβ42. Cells were plated into 6-well plate and treated with CuONP or L-BSO in 1.5 mL media: 6 h on H4 cells, and 16 h on SH-SY5Y cells. Media were harvested and spun down. The supernatants were saved for ELISA assay. For assay, samples were mixed with antibody according to the protocol and incubated overnight. Substrates were added into the plate and read with luminescent reader. The percentage of fluorescent changes were calculated. * p < 0.05, ** p < 0.01.