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Implication of TGF-β signaling pathway in low cyclophosphamide activity. (A) ELISA analysis for serum TGF-β1; (B–D) qPCR analysis for mRNA expression of TGFβRI, TGFβRII, and TGFβRIII, respectively. Serum was extracted after blood collection and tumors were collected; total RNA was isolated, then qPCR was performed, as previously described, *p < 0.05.
