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. 2020 Jan 31;21(3):957. doi: 10.3390/ijms21030957

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of metronomic cyclophosphamide on tumor-associated fibroblasts (TAFs). (A) Immunohistochemistry of FSP-1 in the tumor microenvironment, scale bar = 20 μm. (B) qPCR analysis for the mRNA level of FSP-1 in the tumor microenvironment. (C) Immunohistochemistry of α-SMA in the tumor microenvironment α-SMA, scale bar = 20 μm. (D) qPCR analysis for the mRNA level of α-SMA in the tumor microenvironment. H&E staining was performed as indicated above. Tumors were collected and total RNA was isolated; then, qPCR was performed as previously described.