Figure 3.

Lentiviral delivery of chimerized designer epigenome modifiers (DEMs). (a) Schematic of the designer epigenome modifiers harboring a chimerized TALE DNA binding domain. Lentiviral vectors containing either an active or inactive (lacking the DNA binding domain) DEM were generated and used to transduce HEK293T cells. (b) Activity of chimerized DEM. Cells were transduced with increasing doses of the lentiviral vectors described in a and CXCR4 expression was measured at different time points after transduction via flow cytometry. The histogram shows the extent of CXCR4+ cells (mean ± SEM) in samples transduced with lentiviral vectors encoding for an active DEM relative to those receiving the mock vector (containing a non-targeted DEM lacking the DNA binding domain). Fold change values relative to samples transduced with the mock LV are reported within the bar graph.