FIG 2.

The combination of mefloquine and mefloquine derivatives with fluconazole is fungicidal against Cryptococcus neoformans. (A) The combination of fungistatic concentrations of mefloquine (at one-quarter the MIC) and fluconazole (at one-half the MIC) is fungicidal for C. neoformans strain H99 incubated in YPD at 37°C. (B) All of the mefloquine derivatives except for 13480 showed similar fungicidal activity in combination with fluconazole under the conditions described above. Samples of each culture were plated on YPD at the indicated time points, and the numbers of CFU per milliliter were quantified using serial dilutions after incubation at 30°C. The curves represent the results of single experiments representative of those of at least two independent experiments. Error bars indicate the standard deviations from two technical replicates.