FIG 7.

Mefloquine derivatives dissipate the proton motive force across the mitochondrial membrane. (A) Candida albicans strain SC5314 cells were treated with the indicated concentration of MEF derivative 13480 or DMSO for 2 h in YPD at 37°C. The cells were harvested, stained with either MitoTracker Red CMXRos or MitoTracker Green, and imaged. Identical exposure settings were used for both samples. Propidium iodide staining indicated that >95% of the cells were viable at the time of imaging. (B) Cryptococcus neoformans strain H99 cells were processed as described in the legend to panel A but for MitoTracker Red staining only. (C) Treatment with the other mefloquine derivatives showed a decreased uptake of MitoTracker Red by Candida albicans strain SC5314.