FIG 4.

Unidirectional synergy between chelators and TS. Surface areas of 3D checkerboards were plotted against chelator concentration in terms of percentage of control on a log₁₀ scale and compared to the activity of each chelator alone: DOXY (A), CO (B), and CLI (C). As shown in Fig. S4 in the supplemental material, CLI antagonized with DSX against PA14; thus, the triple combination allowed more growth than CLI alone at its highest concentration. However, the triple combination reduced growth below the previously established MIC, 20% of control (20). (D) TRO. (E) Surface areas were graphed with respect to increasing TS concentrations and compared to the activity of TS alone. n.s., not significantly different; *, P < 0.05; **, P < 0.005; ***, P < 0.0005. The averages from at least three biological replicates are shown.