TABLE 3.
Primers used in this study
| Primer | Sequence (5′→3′) | Notea |
|---|---|---|
| cidA-up-F | GGGGACAAGTTTGTACAAAAAAGCAGGCTATTCTTACCGATGCAGGTCAA | attb1 |
| cidA-up-R | CGGAATTCCGGACCCACGTAATGATGCGCCT | EcoRI |
| cidA-down-F | CGGAATTCCGAATCCAGCATTAATTGCATCT | EcoRI |
| cidA-down-R | GGGGACCACTTTGTACAAGAAAGCTGGGTTTGGCATCATACATACCATTT | attb2 |
| psmα-up-F | GGGGACAAGTTTGTACAAAAAAGCAGGCTTATGGCTAGCACAATACAATC | attb1 |
| psmα-up-R | CGGAATTCCGATTCTCAGGCCACTATACC | EcoRI |
| psmα-down-F | CGGAATTCCGAGATTACCTCCTTTGCTTATG | EcoRI |
| psmα-down-R | GGGGACCACTTTGTACAAGAAAGCTGGGTAGACTCACGTGGCACTTTCC | attb2 |
| gyrB-F | GGTGGCGACTTTGATCTAGC | |
| gyrB-R | TTATACAACGGTGGCTGTGC | |
| RT-agrA-F | TCCAGCAGAATTAAGAACTCG | |
| RT-agrA-R | ATATCATCATATTGAACATACACT | |
| RT-SarA-F | AAACCCTGAATTTGAATG | |
| RT-SarA-R | GATATTACATCTGCTCCT | |
| RT-icaA-F | CTTGGATGCAGATACTATCG | |
| RT-icaA-R | GCGTTGCTTCCAAAGACCTC | |
| RT-icaC-F | GGGAATCCAATTTCTCTTT | |
| RT-icaC-R | ATACAATGACAGCAGACT | |
| RT-cidA-F | TGTACCGCTAACTTGGGTAGAAGAC | |
| RT-cidA-R | CGGAAGCAACATCCATAATACCTAC | |
| RT-atlA-F | GGTGACACTCGTGCTAAT | |
| RT-atlA-R | AGGGCATGTGAGATAAGA | |
| RT-fnbA-F | ATGGAACGAATGGAACAATAAC | |
| RT-fnbA-R | GTGTGGTAATCAATGTCAAGAG | |
| RT-fnbB-F | CTGACGGTGCCGTAGTTC | |
| RT-fnbB-R | GTCGCCTTTAGCGTTATT | |
| RT-lrgB-F | CATCGGAGGTATTGGTATCG | |
| RT-lrgB-R | GTAGTTGCTGCTTGAGGTAA | |
| RT-bap-F | ATATGAACGCCAAGTATC | |
| RT-bap-R | ACAACGGTATCTATATTAGTAA | |
| RT-psmα-F | GTCCTCCTGTATGTTGAT | |
| RT-psmα-R | AATTCACTGGTAAGTAAGTTAT |
a
The underlined sequences represent the BP reaction sites or restriction enzyme sites.