FIG 6.

Vancomycin treatment reduced potentiated hypoxic-ischemic brain injury in S. epidermidis-infected animals. Hypoxia-ischemia was induced 14 h after infection of PND4 mice with S. epidermidis. At the time of infection, mice were injected with saline (n = 21), vancomycin (n = 30), or vancomycin with pentoxifylline (n = 30). Brains were harvested 9 days later (on PND14), stained for microtubule-associated protein 2 or myelin basic protein, and analyzed for tissue loss in the ipsilateral hemisphere. Shown are percentages of gray matter (A) and white matter (B) tissue loss. Box-and-whiskers plots with the box extending from the 25th to 75th percentiles and the medians (line) are depicted. The whiskers indicate the minimum and maximum values. The data were pooled from 6 independent experiments. Statistical analysis comparing different treatment groups employed the Kruskal-Wallis test with Dunn’s multiple-comparison post hoc test (*, P < 0.05; **, P < 0.01; ***, P < 0.001).