FIG 4.

Lead compounds demonstrate synergy with polymyxin B against diverse polymyxin-resistant Gram-negative bacterial species. The effectiveness of PMB and the lead compounds against various polymyxin-resistant Gram-negative bacteria was determined via a PMB Epsilometer test method using CAMHA supplemented with 20 μM of compound. The heat map shows the fold reductions in the MIC of PMB for the various strains tested (data for calculations shown in Table S3). Representative data from at least two biologic replicates are provided.