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. 2020 Feb 21;64(3):e01789-19. doi: 10.1128/AAC.01789-19

FIG 4.

FIG 4

Performance of the CRISPRi system in reducing the class 1 integron-associated HGT of ARGs in E. coli. (A) Schematic diagram of a CRISPRi system to inhibit HGT of ARGs related to the IntI1 integrase-mediated integration of ARG cassettes. (B and C) Transcription of intI1 in the recombinant E. coli harboring plasmid R388, recombinant plasmid plv-dCas9-R(0–6), and either pINT-aadA1 (B) or pINT-aadB (C). (D and E) HGT rates of aadA1 (D) or aadB (E) associated with the IntI1 integrase-mediated integration of ARG cassettes with/without aTc induction. (F and G) Correlation between intI1 transcription and the HGT rate of aadA1 (F) or aadB (G). HGT, horizontal gene transfer; ARGs, antibiotic resistance genes; aTc, anhydrotetracycline; R(0–6), E. coli C600[R388/plv-dCas9-R(0–6)/pINT-cassette]. All data represent the means ± standard deviations of biological triplicates. **, P < 0.01; ***, P < 0.001.