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. 2020 Feb 21;64(3):e01789-19. doi: 10.1128/AAC.01789-19

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Copyright © 2020 Li et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 6 — Reversibility and hereditary stability of a CRISPRi system in E. coli. (A to C) Reversibility of transcriptional levels of the drfB2 cassette (A), sul1 (B), or intI1 (C) in different generations of recombinant E. coli harboring a CRISPRi vector exposed to aTc or not. (D) Hereditary stability and PCR analysis of recombinant plasmid plv-dCas9-R3 serially passaged in liquid culture with or without CM. The strain without exposure to CM was used as the control. aTc, anhydrotetracycline; CM, chloramphenicol; R0, E. coli C600(R388/plv-dCas9-R0); R3, E. coli C600(R388/plv-dCas9-R3). NS, not significant (P > 0.05); ***, P < 0.001.